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Title: Electrospun multiscale fibrous scaffolds for tissue engineering
Researcher: Shalumon, K T
Guide(s): Jayakumar, R
Chennazhi, Krishnaprasad
Keywords: Nanosciences
Molecular Medicine
tissue engineering
Upload Date: 20-Dec-2012
University: Amrita Vishwa Vidyapeetham (University)
Completed Date: July, 2012
Abstract: The use of Electrospun ECM-mimicking nano fibrous scaffolds for tissue engineering is limited by poor cellular infiltration. We hypothesized that cell penetration could be enhanced in scaffolds by using a hierarchical structure where nano fibers are combined with micron-scale fibers while preserving the overall scaffold architecture. To assess this, we fabricated electrospun poly(caprolactone) (PCL) and porous poly(lactic acid) (PLA) scaffolds having nanoscale, microscale and combined micro/nano architecture with and without nano-Hydroxy Apatite (nHAp). All the scaffolds were evaluated for its spectroscopic, morphological, mechanical, thermal, cell attachment and protein adsorption properties. The cell activity, proliferation and total protein adsorption on the nanofibers/ nano-fibers with nHAp was significantly higher than on the micro-fibers although the adsorption per unit area was less on the nano-fibers due to the much higher surface area of nano-fibers. Though the bioactivity was intermediate to that for nanofiber and microfiber scaffold, a unique result of this study was that the micro/nano combined fibrous scaffold showed improved cell infiltration and distribution than the nanofibrous scaffold. While the cells were found to be lining the scaffold periphery in the case of nanofibrous scaffold, micro/nano scaffolds had cells dispersed throughout the scaffold. Further, as expected, the addition of nanoparticles of HAp improved the bioactivity though it did not play a significant role in cell penetration. Thus, this strategy of creating a 3D micro/nano architecture, which would increase the porosity of the fibrous scaffold and thereby improving the cell penetration, can be utilized for the generation of functional tissue engineered constructs in vitro.In the second part of the study, we discussed in detail about the development of tubular scaffolds for vascular tissue engineering.
Pagination: 168p.
Appears in Departments:Amrita School of Engineering

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01_title.pdfAttached File152.73 kBAdobe PDFView/Open
02_certificates.pdf184.69 kBAdobe PDFView/Open
03_table of contents.pdf31.62 kBAdobe PDFView/Open
04_acknowledgemetns.pdf19.98 kBAdobe PDFView/Open
05_dedication.pdf9.48 kBAdobe PDFView/Open
06_abstract.pdf19.37 kBAdobe PDFView/Open
07_abbreviations.pdf70.87 kBAdobe PDFView/Open
08_list of figures.pdf74.55 kBAdobe PDFView/Open
09_list of tables.pdf9.45 kBAdobe PDFView/Open
10_chapter 1.pdf1.64 MBAdobe PDFView/Open
11_chapter 2.pdf1.35 MBAdobe PDFView/Open
12_chapter 3.pdf23.82 MBAdobe PDFView/Open
13_chapter 4.pdf35.6 kBAdobe PDFView/Open
14_chapter 5.pdf153.73 kBAdobe PDFView/Open

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