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Title: Green Synthesis of Silver Nanoparticles using Tinospora cordifolia Extract and Evaluation of Its Antimicrobial Activity
Researcher: Anuj, S.A.
Guide(s): Gajera, H.P.
Keywords: Bactericidal efficacy
Life Sciences,Microbiology,Microbiology
Membrane permeability
Silver nanoparticles
Zeta potential
University: RK University
Completed Date: 2019
Abstract: Materials and Methods: In the present study, bactericidal assessment of silver nanoparticles with different antibiotics was determined against emerging Bacillus megaterium MTCC 7192 and re-emerging Escherichia coli MTCC 443, Pseudomonas aeruginosa MTCC 741, and Staphylococcus aureus MTCC 3160 pathogenic bacteria by agar well diffusion method. Interaction of silver nanoparticles towards bacterial membrane was carried to understand the probable bactericidal actions of silver nanoparticles, which was further confirmed by respiratory chain dehydrogenases, Zeta potential, Scanning Electron Microscopy (SEM) and Gas Chromatography- Mass Spectrometry (GC-MS). newline newlineResults and Discussion: In this work, we observed genuine bactericidal property of silver nanoparticles as compare to broad spectrum antibiotics against emerging and re-emerging mode. The binding affinity of silver nanoparticles towards bacterial membrane induced loss of catalytic activity for respiratory chain dehydrogenases. After being exposed to nanoparticles, the membrane becomes scattered from their original ordered arrangement based on SEM observation. Moreover, our results also suggested that alternation of zeta potential enhanced membrane permeability, and beyond a critical point, it leads to cell death. The leakages of intracellular constituents were confirmed by GC MS. newline newlineConclusions: The present findings suggested that silver nanoparticles directly interact with the bacterial cell surface without the need to penetrate; and this distinctive property raises the hope that silver nanoparticles will remain an important bactericide in bacteria than antibiotics newline
Appears in Departments:Faculty of Science - Applied Mathematics

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02_certificate.pdf313.56 kBAdobe PDFView/Open
03_declaration.pdf381.42 kBAdobe PDFView/Open
04_acknowledgement.pdf383.17 kBAdobe PDFView/Open
05_table of contents.pdf127.48 kBAdobe PDFView/Open
06_list of tables.pdf190.2 kBAdobe PDFView/Open
07_list of figures.pdf209.17 kBAdobe PDFView/Open
08_list of abbreviations.pdf189.54 kBAdobe PDFView/Open
09_abstract.pdf279.01 kBAdobe PDFView/Open
10_graphical abstract.pdf443.82 kBAdobe PDFView/Open
11_chapter 1.pdf642.37 kBAdobe PDFView/Open
12_chapter 2.pdf483.79 kBAdobe PDFView/Open
13_chapter 3.pdf84.63 kBAdobe PDFView/Open
14_chapter 4.pdf382.35 kBAdobe PDFView/Open
15_chapter 5.pdf5.04 MBAdobe PDFView/Open
16_chapter 6.pdf716.37 kBAdobe PDFView/Open
17_chapter 7.pdf296.78 kBAdobe PDFView/Open
18_chapter 8.pdf181.14 kBAdobe PDFView/Open
19_chapter 9.pdf84.19 kBAdobe PDFView/Open
20__list of publication.pdf288.6 kBAdobe PDFView/Open
21_references.pdf377.21 kBAdobe PDFView/Open

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