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|Title:||Phytochemical And Chromatographic Studies Of Indigenous Medicinal Plant Leptadenia Reticulata|
Physical Sciences,Chemistry,Polymer Science
|Abstract:||quotBackground: Leptadenia reticulata, the most important medicinal plant reported for many biological activities. It s mainly known for lactogenic effect and night blindness. L. Reticulata is commonly known as Jivanti, Dori, Dodi. Many formulations of jivanti are available in market. Phytochemical studies of this plant is lacking till date. newlineAim: Objective of the study was to screen plant Leptadenia reticulata by phytochemical and chromatographic studies. To standardize fraction and isolate major phytoconstituents with use of chromatographic techniques. Characterize isolated compound with spectroscopy technique and check activity guided bioactivity of obtain extracts and fractions. newlineMaterials and Methods: For qualitative test in different extract and physicochemical parameters, different tests were performed as per Indian pharmacopeia. Extraction and chromatographic isolation method were developed for sterols, triterpenoids, Pregnane glycosides, Flavonoid by using thin layer chromatography (TLC) and column chromatographic technique. Chromatographic fingerprint profile of L. reticulata areal parts developed by TLC and high-performance thin layer chromatography (HPTLC). Antioxidant and antimicrobial activity were performed on successive extracts and fractions. newlineResults and Discussion: In non-polar part, there are seven compounds were isolated and identify i.e. Hentriacontane, Phytol, Beta amyrin, Lupeol, Campesterol, Stigmasterol, Gamma sitosterol were isolated through fractionation. In glycosides study, earlier three pregnane glycosides were reported while we isolate and identify four novel pregnane glycosides by different spectroscopic methods like, MALDI TOF-MS, 1H NMR, 13C NMR, HMBC, HSQC co-relation. Chromatographic fingerprinting profile was developed for different extracts and fractions of L. reticulata areal parts for standardization. Tentatively identification of Kaempferol and apigenin were done by HPTLC. Activity guided isolation performed on different extract, successive extract and fraction from that resid|
|Appears in Departments:||Faculty of Science - Applied Mathematics|
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