Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/244235
Title: Evaluation of the Role of Extracellular Matrix Protein Tenascin C in Oral Potentially Malignant Disorders and Squamous Cell Carcinoma An Immunohistochemical and Cell Culture Study
Researcher: Deepa R Mane
Guide(s): Dr. Alka D Kale
Keywords: 
University: KLE University
Completed Date: 
Abstract: newline Background: Oral squamous cell carcinomas (OSCC) are a diverse cluster of neoplasm s. In India, an indiscriminate consumption of tobacco is the predominant causative factor for OSCC and most are preceded by potentially malignant disorders (OPMD s). Dysplasia grading in predicting the malignant potential is gold standard, but remains to have disagreements. Hence it s time for oncobiologists to search for reliable marker to predict malignant potential and cancer progression. One such novel ECM molecule is Tenascin-C (TN-C), with multifunctional role and shown to have promising clinical results in systemic malignancies. Minimal research is focused on TN-C in OPMD s and OSCC. Hence our study intended to conduct standard invitro study to translate Tenascin-C from bench to bed side. newlineAim and Objectives: newlineAim: Evaluation of the role of TN-C in Potentially Malignant disorders and Oral Squamous Cell Carcinoma by Cell Culture and Immunohistochemical methods. newlinePrimary objective: To evaluate the role of TN-C for its effect on cell migration, invasion and proliferation of OSCC cell line. newlineSecondary objectives: To evaluate the immunoexpression of Tenascin-C in oral potentially malignant disorders newlineMethodology: All our cases are from Indian origin with predominant chewing tobacco habit; hence we performed cell cultures assays of cell migration, invasion and proliferation on standard authenticated Indian patient derived OSCC cell line AW13516, etiology being gutkha chewing. The presence of TN-C was confirmed by flow cytometry and all the cell culture assays were performed with stringent protocols. After sample size calculation 550 cases of OPMDs and OSCC cases were subjected to immunohistochemistry to determine the immunoexpression of TN-C. Interpretation of IHC was assessed manually and also quantified by ImageJ software. Statistical tests of ANOVA, Chi-Square and sensitivity and specificity was computed, at 95% CI and p and#8804; 0.05 to be statistically significance. newlineResults: Flow cytometry showed 91.13% intracellular and 20.89% surface expression of TN-C in AW13516 cell line. Gain of function and loss of function of TN-C was evident in both cell migration and invasion assays with statistical significance but the same was not depicted in proliferation assays. We noted that positive expression of TN-C in all PMD s found to have gt80% sensitivity and specificity for identification of active stromal remodeling by TN-C. Moreover TN-C positive expression showed 89% sensitivity and 87.8% specificity for prediction of metastasis. newlineConclusion: Our study is first of its kind to demonstrate proper bench study regarding TN-C in OPMDs and OSCC of Indian Patients. We would like to hypothesize that TN-C has a significant role in promoting migration and invasion in Indian patient derived cell line. Furthermore its expression revealed good sensitivity and specificity in predicting stromal remodeling and metastasis. Hence future scope of our study is to present our bench data to pharmacological companies to produce anti-TN-C antibody against cases of OPMDs and OSCC for further clinical approach on Indian Patients newline
Pagination: 
URI: http://hdl.handle.net/10603/244235
Appears in Departments:Faculty of Dentistry

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0.certificates.pdfAttached File256.44 kBAdobe PDFView/Open
10. limitations and future scope.pdf22.87 kBAdobe PDFView/Open
11. bibliography.pdf151.61 kBAdobe PDFView/Open
12. annexures.pdf691.4 kBAdobe PDFView/Open
1. introduction.pdf36.68 kBAdobe PDFView/Open
2. aims & objectives.pdf33.34 kBAdobe PDFView/Open
3. review of literature.pdf1.02 MBAdobe PDFView/Open
4. materials and methods.pdf421.25 kBAdobe PDFView/Open
5. data analysis plan formatted.pdf21.07 kBAdobe PDFView/Open
6. results.pdf746.85 kBAdobe PDFView/Open
7. photomicrographs.pdf783.45 kBAdobe PDFView/Open
8. discussion.pdf259.69 kBAdobe PDFView/Open
9. summary and conclusion.pdf37.54 kBAdobe PDFView/Open


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