Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/227584
Title: Isolation production purification and characterization of thermostable bacterial superoxide dismutase
Researcher: Bhatia,Kavita
Guide(s): Seth, Amit
Keywords: Life Sciences,Microbiology,Biotechnology and Applied Microbiology
University: Shoolini University of Biotechnology and Management Sciences
Completed Date: 2017
Abstract: newlineviii newlineABSTRACT newlineThe present study was conducted with the aim of isolating superoxide dismutase producing thermophilic bacteria from water samples of different hot water springs of Himachal Pradesh. One isolate from Manikaran named as M560 exhibited maximum superoxide dismutase activity out of seventy isolates. This isolate was identified, based on morphological and biochemical characteristics and also by molecular characterization. 16S rRNA sequencing results showed that the isolate was Anoxybacillus gonensis. In order to enhance the activity of SOD (superoxide dismutase), response surface methodology (RSM) based on central composite design was used. newlineFurther superoxide dismutase of Anoxybacillus gonensis was purified to homogeneity. A purification factor of 33.1-fold was achieved, with the purified enzyme exhibiting specific activity of 5758.4 U/mg protein. The molecular weight of superoxide dismutase was calculated as 31 kDa by SDS-PAGE. The native-PAGE analysis showed that superoxide dismutase of Anoxybacillus gonensis exists as homodimer (62 kDa). Inhibition studies on purified superoxide dismutase showed that it was Cu/Zn type superoxide dismutase. This is the first report ever showing purification of superoxide dismutase from Anoxybacillus gonensis. newlineA gene encoding superoxide dismutase of approximately 491 bp was also amplified using genomic DNA of Anoxybacillus gonensis KA 55. In silico docking studies have shown which amino acid residues were involved in forming hydrogen bond and hydrophobic interactions with various inhibitors. Keywords: Superoxide dismutase, Anoxybacillus gonensis, 16S rRNA, Response Surface Methodology, In silico, Hydrophobic, Inhibitors
Pagination: 171p,
URI: http://hdl.handle.net/10603/227584
Appears in Departments:Faculty Of Biotechnology

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10figures.pdfAttached File334.2 kBAdobe PDFView/Open
11 abstract.pdf152.52 kBAdobe PDFView/Open
12chapter-1.pdf600.91 kBAdobe PDFView/Open
13 chapter 2 review.pdf1.13 MBAdobe PDFView/Open
14 chapter3 materials.pdf771.02 kBAdobe PDFView/Open
15 chapter 4 results.pdf5.43 MBAdobe PDFView/Open
16 chapter 5 discussion.pdf414.53 kBAdobe PDFView/Open
17 chapter 6 summary.pdf385.46 kBAdobe PDFView/Open
18 chapter 7 future.pdf242.62 kBAdobe PDFView/Open
19 chapter 8 reference.pdf475.85 kBAdobe PDFView/Open
1title page (1).pdf197.5 kBAdobe PDFView/Open
20 appendices.pdf338.42 kBAdobe PDFView/Open
21 publications.pdf265.94 kBAdobe PDFView/Open
22first paper kavita.pdf3.75 MBAdobe PDFView/Open
23second paper.pdf2.74 MBAdobe PDFView/Open
24kavita ami.pdf218.19 kBAdobe PDFView/Open
2declaration.pdf230.42 kBAdobe PDFView/Open
3 certificate 1.pdf234.44 kBAdobe PDFView/Open
4 certificate 2.pdf233.63 kBAdobe PDFView/Open
5 certificate 3.pdf463.2 kBAdobe PDFView/Open
6.5 dedication page.pdf260.34 kBAdobe PDFView/Open
6 content.pdf500 kBAdobe PDFView/Open
7 acknowledgement.pdf242.85 kBAdobe PDFView/Open
8list of abbreviations.pdf260.68 kBAdobe PDFView/Open
9 list of tables final.pdf314.67 kBAdobe PDFView/Open


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