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Title: Sequential microbial photocatalytic process for degradation of neonicotinoid pesticide
Researcher: Sharma, Teena
Guide(s): Rajor, Anita and Toor, Amrit Pal
Keywords: Life Sciences,Microbiology,Biotechnology and Applied Microbiology
University: Thapar Institute of Engineering and Technology
Completed Date: 2015
Abstract: Imidacloprid (IMI) insecticide was selected from the neonicotinoid family on the basis of its intensive usage throughout the year for wheat and paddy crop in Punjab (India). Soil samples for isolation of bacteria were collected from paddy field and five bacterial strains (T1-T5)were isolated on enrichment media (NB) containing 50 mgL-1 of IMI. Out of these five, strain T1 and T5 exhibited the highest growth and further identified/characterized on the basis of morphological, biochemical, physiological characterization and phylogenetic analysis using 16S rRNA sequencing. Results showed that both of the isolates are a member of Enterobacter sp. and hereafter mentioned as ATA1 and ATA2. Phylogenetic tree were constructed for both isolates by neighbour joining method using GenBank-NCBI database. A close similarity of strain ATA1 was confirmed with Enterobacter asburiae JCM6051 (AB004744) with 98.1% gene sequence whereas strain ATA2 has shown 98% similarity with Enterobacter hormaechei ATCC 49162 strain (AZ508302). Both isolates were referred as Enterobacter sp. ATA1 and Enterobacter sp. ATA2. A significant growth shown by strain ATA1 as compared to strain ATA2 was further selected for degradation studies. Initially, tolerance to IMI at various concentrations (50-150 mgL-1) in minimal media was performed and observed more growth for 50 mgL-1 as compare IMI=100 mgL-1 and 150 mg L-1. Various co-metabolites (Maltose, Sucrose, Fructose, Lactose and Glucose) were used as additional source of carbon for both growth and degradation studies in minimal media broth. Among, these studied co-metabolites, glucose (0.1% w/v) found to show maximum growth, indicated its effective use as carbon source than the others. Co-metabolite degradation studies were performed in minimal media with glucose (MMG 0.1%) under the optimized concentration of IMI (50 mgL-1) and glucose (0.1% w/v) and degradation of ~45% was confirmed at 3rd of incubation. No notable change was observed upto 15 days of degradation time.
Pagination: xxv, 158p.
Appears in Departments:Department of Biotechnology

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