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Title: Search for Novel Bioactives as Lead Therapeutis from Actinomycetes
Researcher: Meeta Masand
University: Suresh Gyan Vihar University
Completed Date: 2018
Abstract: newline The uphill in the drug-resistant microbial pathogens have made many newlineexisting antibiotics as obsolete. This sharp rise in the emergence of multi-drug newlineresistant pathogens needs to be checked by the use of new therapeutic drugs. This newlinehas created a need to unfold and bring into scene novel therapeutic drugs that can newlinecure the disease caused by multi-drug resistant pathogens. The retort to this urgent newlineneed incorporates new attention towards a group of actinobacteria, generally known newlineas actinomycetes (order Actinomycetales), that have been well accepted as the rich newlinesource of natural products covering polyketides, non ribosomal peptides and their newlinecombinations too. Natural products from the actinomycetes are an excellent source newlineof antibiotics. newlineConsidering this in account, the current research was initiated with an newlineobjective to search novel strains and study the antimicrobial efficacy of the newlineactinomycetes isolates. Actinomycete strains were isolated from the soil samples of newlinethe poorly studied environments including semi arid regions of Rajasthan. Different newlinephysical and chemical pretreatment methods were employed for the selective newlineisolation of slow growing actinomycetes including drying and addition of newlineantibacterial and antifungal antibiotics in media and stamping on specific media like newlinestarch casein nitrate agar, actinomycetes isolation agar commonly used for the newlineisolation of actinomycetes. newlineAn amalgamation of all such methods aided the isolation of filamentous newlineactinomycetes with a concurrent abatement of other bacterial and fungal newlinecontaminations. A total of twenty one strains were carefully selected and newlinesubcultured several times to attain the pure colonies. newlineAll the twenty one strains were screened using cross streak method as the newlinepreliminary test against the bacterial strains Pseudomonas aeruginosa ATCC 10145, newlineEscherichia coli ATCC 3739, Methicillin-resistant Staphylococcus aureus (MRSA) newlineand Vancomycin-resistant Enterococcus (VRE) and a yeast Candida albicans. All newlinethe twenty one strains were subjected to seco
Appears in Departments:Department of Science

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acquisition method(1) - cutted (2).pdf1.03 MBAdobe PDFView/Open
bibliography - cutted.pdf430.24 kBAdobe PDFView/Open
conclusion - cutted.pdf166.68 kBAdobe PDFView/Open
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front pages (1) - cutted.pdf486.13 kBAdobe PDFView/Open
introduction - cutted.pdf182.56 kBAdobe PDFView/Open
material & method - cutted.pdf618.03 kBAdobe PDFView/Open
tables (1) - cutted.pdf24.1 kBAdobe PDFView/Open

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