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Guide(s): Hardik Pathak
University: JECRC University
Completed Date: 01/02/2018
Abstract: newline Petroleum compounds have been used for many decades for illumination and on a smaller scale, as a lubricant. Today s soil pollution by petroleum hydrocarbons has become a serious problem the world. It is caused by petrol and its products which have been proved as a great disaster on the ecosystem. Bioremediation is one of the most economical methods which is used to treat this threat and an approach to save the environment from petroleum related issues. newlineThe petroleum contaminated soil samples were collected from four different motor garage and service station of Churu (Arid region of Rajasthan). Contaminated soil samples were collected in sterile poly bags and stored in the laboratory for the study. Physical and Chemical properties of contaminated and Normal soil samples like pH, Soil texture, Water holding capacity, Moisture content, and Heavy metal detection etc. were performed. Total petroleum hydrocarbon extracted through the column chromatography and analysed by GC-MS. Isolation of hydrocarbon utilizing microorganisms was performed by the enrichment culture technique with BH medium. Spread plate method and Streak plate method was used to get the pure colonies. All isolates were characterized morphological and biochemical characterization. After the biochemical and morphological characterization selected 12 isolates were subjected to molecular characterization (16s rRNA sequencing). 11 isolates were characterized as gram negative and only one isolate characterized as Gram positive. After molecular characterization all 12 isolates were identified as Achromobacter xylosoxidans M66, Acinetobacter baumannii DSM 30007, Planomicrobium chinense DX3-12, Stenotrophomonas acidaminiphila AMX 1, Brevibacillus agri NBRC 15538, Alcaligenes faecalis J, Pseudomonas aeruginosa NBRC 12689, Pseudomonas stutzeri CCUG 11256, Pseudomonas chengduensis MBR, Pseudomonas putida NBRC 14164, Streptomyces pseudogriseolus HBUM 173455 and Serratia marcescens NBRC 102204. One fungal species was identified as Streptomyces pseudogriseolus HBUM 173455. newlineBiodegradation potential of all 12 isolates was determined using the gravimetric method. 2T Engine Oil was used as an energy source. The highest newlineviii newlinedegradation of 2T engine oil was observed (62%) by Achromobacter xylosoxidans M66 and Pseudomonas aeruginosa NBRC 12689. 10 isolates were showed the degradation between 40-60%. Analysis of degraded hydrocarbon compounds determines by the GC-MS. In the degradation process, High molecular weight Hydrocarbons were converted into small molecular weight compounds.
Appears in Departments:Department of Biotechnology

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