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Title: In Vitro And Pharmacological Studies on Canthium Coromandelicum Burm F Alston
Researcher: Ekambe Priyadarshini Satishrao
Guide(s): Surwase B. S.
Keywords: Canthium Coromandelicum
University: Swami Ramanand Teerth Marathwada University
Completed Date: 08/01/2017
Abstract: Canthium coromandelicum (Burm. f.) Alston is a medicinal plant belonging to family of Rubiaceae. This plant is used in the treatment of scabies, ringworm, cough, indigestion and snake bite. It shows various pharmacological properties like antioxidant, preliminary anti-inflammatory, antirhuematic, wound healing and diuretic activity. newlineIn the present work, protocols for in vitro seed germination, direct /indirect shoot regeneration and in vitro production of normal root culture were developed. The research work was also carried out to evaluate anti-diabetic, anti-inflammatory and antimicrobial activity of the selected plant. newlineHigh frequency in vitro clonal propagation protocols were standardized from the nodal explants derived from in vitro raised 30 days old seedlings. Knudson C (KC) medium was the best medium for seed germination and seedling development. While, maximum shoot regeneration frequency through callus was obtained on MS (Murashige and Skoog medium)+ 4 mg/l BAP (6-benzyl amino purine)+ 1 mg/l IAA (Indole -3- acetic acid), optimum direct shoot multiplication frequency was obtained on MS (Murashige and Skoog medium)+ 3 mg/l Kn ( Kinetin)+ 1 mg/l IAA (Indole -3- acetic acid). The elongated shoots were sub cultured on ¼ MS with 3 mg/l NAA for rooting. The in vitro raised plantlets were acclimatized in green house and progressively transplanted to natural conditions with 70% survival. newlineOptimum result of fast growing normal root culture using leaf explants under total darkness was obtained on solid ½ MS+2.5 mg/l IBA+1 mg/l NAA with 14.30±1.08 number of roots per culture with 3.00±0.42 gm fresh biomass per culture. In liquid culture, there was maximum of 1.91±0.05 gm dry biomass after fifth week in same medium. In vitro produced fast growing roots showed similar chemical profile like that of natural roots. newlineThe present work was also carried out to evaluate anti-inflammatory and anti-diabetic activity of different crude leaf extracts of Canthium coromandelicum (Burm. f.) Alston. The anti-inflammatory activity of a
Pagination: 144p
Appears in Departments:School of Life Sciences

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01_title.pdfAttached File165.81 kBAdobe PDFView/Open
02_certificate.pdf184.77 kBAdobe PDFView/Open
03_abstract.pdf179.22 kBAdobe PDFView/Open
04_declaration.pdf137.5 kBAdobe PDFView/Open
05_acknowledgement.pdf85.52 kBAdobe PDFView/Open
06_contents.pdf112.36 kBAdobe PDFView/Open
07_list_of_tables.pdf100.34 kBAdobe PDFView/Open
08_ list_of_figures.pdf151.04 kBAdobe PDFView/Open
09_abbreviations.pdf224.32 kBAdobe PDFView/Open
10_chapter 1.pdf107.76 kBAdobe PDFView/Open
11_chapter 2.pdf350.65 kBAdobe PDFView/Open
12_chapter 3.pdf318.52 kBAdobe PDFView/Open
13_chapter 4.pdf2.29 MBAdobe PDFView/Open
14_conclusions.pdf54.81 kBAdobe PDFView/Open
15_bibliography.pdf196.16 kBAdobe PDFView/Open

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