Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/184935
Title: Method Development and Validation for Simultaneous Estimation of Amlodipine besylate and Enalapril maleate Amlodipine besylate and Lisinopril dihydrate Amlodipine besylate and Losartan potassium in tablet dosage form using UV Spectrophotometry HPLC and UPLC
Researcher: Manisha Masih
Guide(s): Abhilasha Mittal
Keywords: 
University: Jayoti Vidyapeeth Women s University
Completed Date: 
Abstract: Simple, sensitive and specific spectrophotometric methods were developed and validated for quantization of Amlodipine besylate, Enalapril maleate, Lisinopril dihydrate and Losartan potassium in tablet dosage form. Analytical methods were developed based on the simultaneous estimation of drugs in a binary mixture without prior separation. In simultaneous equation method, the drugs were determined by using the absorptivity values of Amlodipine besylate, Enalapril maleate, Lisinopril dihydrate and Losartan potassium at selected wavelengths, as per their uv scan spectrum, respectively. The solvents used were methanol: 1N HCl (1:1) and methanol : 0.1N HCl (1:1). newlineA simple, reproducible and efficient high performance liquid chromatographic method was developed for simultaneous determination of Amlodipine besylate, Enalapril maleate, Lisinopril dihydrate and Losartan potassium in tablets dosage form. The chromatographic separation was carried out using Analytical Technologies Ltd HPLC system with UV-2230 UV-Vis detector, P-2230 HPLC pump and a Phenomenex Gemini C18 column (250 × 4.6 mm, 5and#956;m particle size). The isocratic mobile phase consisting of methanol: 1N HCl(1:1) and methanol : 0.1N HCl(1:1).. The flow rate of the mobile phase was 1.0 ml/min. The buffer used are 4.7 g of sodium dihydrogen orthophosphate and 1 ml of triethyl amine in 1000 ml of water and the pH of the solution was adjusted to 4.0 ± 0.05 with orthophosphoric acid. The column temperature was kept ambient and injection volume was 20and#956;l. The developed method was found to be accurate, precise and selective for simultaneous determination of Amlodipine besylate, Enalapril maleate, Lisinopril dihydrate and Losartan potassium in tablets dosage form. newlineA simple, precise ultra-performance liquid chromatography method was developed for the simultaneous quantitative determination of Amlodipine besylate, Enalapril maleate, Lisinopril dihydrate and Losartan potassium from their innovative pharmaceutical combination drug product The chromatographic separation was carr
Pagination: 
URI: http://hdl.handle.net/10603/184935
Appears in Departments:Department of Pharmaceutical Science

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10.mat & methods.pdfAttached File416.97 kBAdobe PDFView/Open
11. results.pdf2.81 MBAdobe PDFView/Open
12. discusssion.pdf352.29 kBAdobe PDFView/Open
13.conclusion.pdf209.64 kBAdobe PDFView/Open
14. references.pdf499.83 kBAdobe PDFView/Open
1. list of contents.pdf206.1 kBAdobe PDFView/Open
5. abstract.pdf224.39 kBAdobe PDFView/Open
6.introduction.pdf665.89 kBAdobe PDFView/Open
7.literature review.pdf556.66 kBAdobe PDFView/Open
acknowledgement.pdf98.06 kBAdobe PDFView/Open
certificate.pdf150.5 kBAdobe PDFView/Open
dedication.pdf61.04 kBAdobe PDFView/Open
title page.pdf135.4 kBAdobe PDFView/Open


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