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Title: A Revolutionary Approach for Inactivated Vaccine Production and Radioprotection using Mn2 Complexes from Deinococcus radiodurans
Researcher: Gayen, Manoshi
Guide(s): Maheshwari, Radha. K.
Keywords: Biological Science, Vaccine Production , Radioprotection, Deinococcus radiodurans
University: Birla Institute of Technology and Science
Completed Date: 2015
Abstract: The unparralled resistance to radiation toxicity in ionizing radiation resistant bacterium Deinococcus radiodurans, is directly linked to the intracellular accumulation of divalent manganese ions (Mn2+), which forms complexes with other small metabolites. These Mn2+ complexes have exceptional free radical (ROS) scavenging capability and confer protection to proteins from radiation incurred oxidative damage. The predominant components of these Mn2+ complexes are Mn2+, orthophosphate and peptides. In vitro reconstituted Mn2+ complexes (MDP) at approximately its cytosolic concentrations in D. radiodurans, preserve the enzymatic activity of irradiated enzymes even at 50 kGy. These findings indicated that in vitro reconstituted Mn2+ complex possess its ROS scavenging property. Its protection against oxidative damage has also been extended to the irradiation of Human Jurkat T cells upto 100 Gy. Venezuelan equine encephalitis virus (VEEV) and chikungunya virus (CHIKV) are important alphaviruses from military as well as global health perspective. VEEV has a potential bioweapon status and causes high mortality in aerosolized form. CHIKV epidemics have recently wreacked rampage throughout the Southeast Asian continent and Indian Ocean islands. CHIKV also spread to Europe, Carribean islands and Americas for the first time since the discovery of the virus. Both these alphaviruses lack FDA approved vaccines and specific antiviral therapeutic intervention. In our study we have harnessed the ROS scavenging property of the MDP complexes to generate highly immunogenic inactivated vaccines for these alphaviruses. Irradiation of whole viruses in presence of these complexes to supralethal dose of 50 kGy has generated highly immunogenic and noninfectious virus preparations. The inactivated vaccine also conferred protection against lethal wild type challenge in mouse model. Nuclear warfare and terrorism is a red alert concern globally. Decades of radiation biology research has yielded few promising leads but none provides complete pro
Pagination: 200p.
Appears in Departments:Biological Science

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