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Title: Molecular Characterization of ACC Deaminase Producing Bacteria for Induced Systemic Tolerance in Wheat Triticum aestivum L
Researcher: Singh, Rajnish Prakash
Guide(s): Jha, Prabhat Nath
Keywords: Biological Science, Tolerance in Wheat, Triticum aestivum L
University: Birla Institute of Technology and Science
Completed Date: 2016
Abstract: Plant growth and productivity are highly affected by the several environmental newlinestress factors. Among different plant responses to stressors, increase in the pool of ACC (1-aminocyclopropane-1-carboxylic acid), an immediate precursor of ethylene, leads to the production of stress ethylene that severely affects the various parameters of plant growth and yield. Plant growth promoting associative/endophytic bacteria with ACC deaminase activity can hydrolyze the ACC to and#945;-ketobutyric acid and ammonia and maintain the optimum level of ACC inside the plants which in turn stimulates plant growth under stress conditions. These PGPR can also promote plant growth by providing nutrition (nitrogen, iron, and phosphorous), phytohormone production, and amelioration of stressors by inducing production of osmolytes and antioxidants in plants. Several PGPR producing ACC deaminase has been reported to promote plant growth under stress conditions. However, the detail characterization of these bacteria at molecular level and their molecular effect on plant health are scarce. Moreover, few studies have reported isolation of ACC deaminase producing bacteria from plants of desert region which faces extremes of stresses namely temperature and salinity. Therefore, the aim of the present study was to investigate and characterize the plant growth promoting rhizobacteria (PGPR) with ACC deaminase activity at physiological newlineand molecular level to exploit its effect on plant (wheat) under stress conditions, particularly salt and temperature. Major objectives of the present study were: (i) isolation of associative ACC deaminase producing bacteria from plants growing in Thar desert and their characterization of plant growth promoting properties, (ii) to test the effect of selected PGPR on growth of wheat plant under salt and temperature stresses, (iii) selection of an efficient newlinestrain based on plant growth promoting effect and to test their ability to ameliorate salt stress through modulation of antioxidants and osmolytes in plants, (iv) generat
Pagination: 238p.
Appears in Departments:Biological Science

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