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Title: Studies on Immuno molecular Pathways Triggered by Chlamydia trachomatis Infection in First Trimester Spontaneous Abortion
Researcher: Singh, Namita
Guide(s): Rastogi, Sangita
Keywords: Biological Science, Immuno-molecular, Chlamydia trachomatis Infection
University: Birla Institute of Technology and Science
Completed Date: 2017
Abstract: Chlamydia trachomatis is the etiology of majority of sexually transmitted diseases. newlineColonization of C. trachomatis in the genital tract during early pregnancy has been newlineassociated with spontaneous abortion in women. Although an association between C. newlinetrachomatis infection and spontaneous abortion is acknowledged in various studies, there is a paucity of mechanistic studies till date on the underlying immunomolecular pathway leading to spontaneous abortion in infected women. Hence the cyclooxygenase derived prostaglandin-signalling pathway along with proinflammatory cytokines, hormones, matrix metalloproteinases and their inhibitors was studied in C. trachomatis-positive spontaneous aborters (SA) for an improved understanding of varied immunomolecular aspects during spontaneous abortion in women infected with C. trachomatis. In the present study, firstly, the molecular detection of C. trachomatis was newlineperformed in the Endometrial Currettage Tissues (ECT) of aborters by using PCR and newlinereal time PCR assays. The present study confirmed that C. trachomatis infection should be taken into consideration as far as pregnancy failures are concerned. The expression of COX-1 and COX-2 genes was further studied in C. trachomatis-positive SA in comparison to C. trachomatis-negative SA and uninfected controls. The expression newlinepattern of COX-1/ COX-2 was also compared between recurrent SA (RSA) in and sporadic SA (SSA). Furthermore, the prostaglandin E/ F/ I receptors (EP-1, EP-2, EP-3,EP-4, FP and IP) were also quantified in the ECT of aborters. Mean serum estrogen and progesterone concentration was also measured in C. trachomatis-positive SA, C. trachomatis-negative SA and controls. Our findings delineated that increased expression newlineof COX-2 and PG receptors particularly the contractile receptors and hormonal imbalance could be a possible risk factor for abortion in C. trachomatis-infected SA. In addition, an inflammatory response was seen in C. trachomatis-infected SA. This was deduced on the basis of significantly increased cytok
Pagination: 174p.
Appears in Departments:Biological Science

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