Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/153670
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dc.date.accessioned2017-06-12T09:24:45Z-
dc.date.available2017-06-12T09:24:45Z-
dc.identifier.urihttp://hdl.handle.net/10603/153670-
dc.description.abstractAdulteration in the medicinal plant material is the prevailing factor posing negative effects on the commercialization of medicinal plants. Both intentional and accidental mixing and substitution of original drug raw material with the less effective species reduces the quality of the final products. Use of authentic and original species is the very first and critical step to produce good quality, reliable and efficacious herbal drugs. Scientific validity of the species is the first and very essential parameter for quality assurance of the plant based therapeutics. newlineMany conventional techniques like macroscopy, microscopy and isolation of chemical marker compounds have been using since long to identify authentic plants and to separate them from their adulterants. Genome based identification methods provide an alternative tool which can be used independently or synergistically with the conventional methods. Amalgamation of DNA based techniques with the conventional techniques of identification present an effective approach for quality control and assurance of medicinal plants. newlineThis project is based on the application of a part of genome that is internal transcribed spacer (ITS) region for the authentication of plant material to ensure the quality of herbal drugs. ITS is one of the proposed barcoding locus for identification of plants. Sequence of ITS region acquires species specific variations as the results of evolutionary processes. This region is flanked with the genes for ribosomal RNA. To carry out the project medicinal plants and their adulterant species were collected from various locations. Two medicinally important plants were selected for the project one is Terminalia arjuna which is a cardioprotectent and can be adulterated with other Terminalia species. Secondly, Convolvulus microphyllus vs Evolvulus alsinoides which have controversial identity due to ambiguous vernacular name Shankhpushpi . ITS region of each was amplified and sequenced. The sequences thus generated were compared to identify spec
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dc.languageEnglish US
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dc.rightsuniversity
dc.titleDEVELOPMENT OF DNA BASESD MARKER FOR UNEQUIVOCAL IDENTIFICATION AND AUTHENTICATION OF MEDICINAL PLANTS WITH SPECIAL REFERENCE TO ADULTERATION
dc.title.alternative
dc.creator.researcherSharma Sonal
dc.subject.keywordAdulteration
dc.subject.keywordADULTERATION
dc.subject.keywordmacroscopy
dc.subject.keywordMEDICINAL
dc.subject.keywordUNEQUIVOCAL
dc.description.noteSCIENCE
dc.contributor.guideSHRIVASTAVA NEETA
dc.publisher.placeAhmedabad
dc.publisher.universityNirma University
dc.publisher.institutionInstitute of Science
dc.date.registered12/02/2011
dc.date.completed06/03/2017
dc.date.awarded20/04/2017
dc.format.dimensions
dc.format.accompanyingmaterialDVD
dc.source.universityUniversity
dc.type.degreePh.D.
Appears in Departments:Institute of Science

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10.chapter 1.pdfAttached File497.44 kBAdobe PDFView/Open
11. chapter 2.pdf259.3 kBAdobe PDFView/Open
12. chapter 3.pdf181.32 kBAdobe PDFView/Open
13. chapter 4.pdf5.56 MBAdobe PDFView/Open
14. chapter 5-summary and conclusion.pdf70.91 kBAdobe PDFView/Open
15. bibliography.pdf148.41 kBAdobe PDFView/Open
1.title page.pdf88.36 kBAdobe PDFView/Open
2. certificate.pdf1.44 MBAdobe PDFView/Open
3. abstract.pdf31.62 kBAdobe PDFView/Open
4. declaration.pdf1.59 MBAdobe PDFView/Open
5. acknowledgement.pdf47.86 kBAdobe PDFView/Open
6. table of content.pdf29.6 kBAdobe PDFView/Open
7. list of table.pdf18.74 kBAdobe PDFView/Open
8. list of figure.pdf29.04 kBAdobe PDFView/Open
9. list of abbreviations.pdf32.22 kBAdobe PDFView/Open


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