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Title: Electrospun nano fibrous matrix with human bone marrow derived mesenchymal stem cells for bone tissue engineering
Researcher: Sathy, Binulal Nelson
Guide(s): Nair, Shantikumar V
Keywords: Nano science
Electrospun nano-fibrous matrix
bone tissue engineering,
marrow derived mesenchymal
electrospun PCL Nano
Micro fibers
Upload Date: 20-Nov-2013
University: Amrita Vishwa Vidyapeetham (University)
Completed Date: 2013
Abstract: Tissue engineering is an interdisciplinary approach which combines cell biology newlineand materials science with the purpose of developing implants using live cells and newlinesynthetic extracellular matrix. This thesis attempts this approach in order to newlinedevelop an alternative for autologous bone grafts. Electrospinning is one of the newlinewidely used techniques to fabricate polymeric fibers mimicking the structural newlinedimensions of extracellular matrix for bone tissue engineering applications. newlineHowever current bone tissue engineering approaches using electrospun nanofibers newlinedemand a construct design to address several major challenges in the field. newlineTherefore this thesis work is focussed on developing a construct design which can newlineaddress challenges such as cell infiltration and vascularisation in the construct, newlinecontrolling the degradability of the electrospun fibers, and the scalability of the newlineconstruct. In the first part of this PhD work we have extensively evaluated newlineelectrospun Poly (and#1028;-caprolactone) (PCL) nano-fibers, micro-fibers and human newlinemesenchymal stem cells (hMSCs) for bone tissue engineering purpose. Our results newlineindicated that hMSCs attached and spread rapidly on nano-fibrous scaffolds in newlinecomparison to micro-fibrous PCL. Further, hMSCs proliferated well on the nanofibrous newlinescaffolds. The cells on the nano-fibrous PCL were found to differentiate newlineinto the osteoblast lineage and subsequently mineralize upon addition of in vivo newlineosteogenic regulators. The attachment and spreading of hMSCs were more newlineeffective on the nano-fibers compared with the micro-fibers despite the lower newlineprotein surface coverage (total adsorbed protein per unit fiber surface area) on newlinenano-fibers. Thereafter, nano-fibrous semi-synthetic polymeric nanocomposite newlinescaffolds were engineered by incorporating a maximum of 15 wt. % biopolymeric newlinegelatin nanoparticles (nG) into the synthetic polymer PCL prior to electrospinning newlinein order to impart degradability to the nano-fibers.
Pagination: xix, 145p.
Appears in Departments:Amrita School of Engineering

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01_title.pdfAttached File183.24 kBAdobe PDFView/Open
02_certificate.pdf172.97 kBAdobe PDFView/Open
03_acknowledgements.pdf204.5 kBAdobe PDFView/Open
04_abstract.pdf201.84 kBAdobe PDFView/Open
05_abbreviations.pdf206.24 kBAdobe PDFView/Open
06_certificate.pdf184.46 kBAdobe PDFView/Open
07_declaration.pdf259.27 kBAdobe PDFView/Open
08_dedication.pdf149.4 kBAdobe PDFView/Open
09_list of figures.pdf163.45 kBAdobe PDFView/Open
10_contents.pdf193.22 kBAdobe PDFView/Open
11_synopsis.pdf814.26 kBAdobe PDFView/Open
12_chapter 1.pdf968.38 kBAdobe PDFView/Open
13_chapter 2.pdf418.93 kBAdobe PDFView/Open
14_chapter 3.pdf4.7 MBAdobe PDFView/Open
15_chapter 4.pdf86.01 kBAdobe PDFView/Open
16_publications.pdf7.56 MBAdobe PDFView/Open

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