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Title: Particulate carriers as drug delivery systems for anti-tubercular and anti-cancer agents
Researcher: Patel, Miteshbhai D
Guide(s): Devarajan, Padma V
Keywords: Pharmacy
Colloidal Drug Delivery Systems
Upload Date: 28-Oct-2013
University: Institute of Chemical Technology
Completed Date: 2012
Abstract: Part I: Design and Evaluation of Polyethylene Sebacate-Anti-tubercular drug Nanoparticles: Conventional therapy for tuberculosis (TB) exhibits serious limitation of toxicity, insufficient bioavailability at the target sites (macrophages) and emergence of multidrug resistance. Nanoparticles enable enhanced drug bioavailability, controlled release, and the possibility of targeting to the site of action (i.e. infected macrophages). Infected macrophages express a number of receptors, amongst these one of the widely investigated receptor is folate receptor. Ligand anchoring with folic acid (FA) could further enhance macrophage uptake. Oral drug administration with high bioavailability, sustained release and enhanced pulmonary uptake represents an ideal drug delivery strategy for the treatment of tuberculosis. The present studies discuss the design and evaluation of PES-Rifampicin (RIF), PES-RIF-Ethambutol (EMB) and PES-RIFMSDNC- 22 nanoparticles with and without folic acid. Poly (ethylene sebacate) (PES) a new biodegradable, non-mutagenic and non-genotoxic polymer was selected for the present study. UV and HPLC methods have been developed and validated for all drugs and drug combinations to enable assay, in vitro release, stability and quantification in presence of biological samples. PES nanoparticles of RIF and RIF-MSDNC-22 were developed and optimized by nanoprecipitation method. RIF-ETH nanoparticles were prepared by multiple emulsion method to enable high entrapment of hydrophilic ETH. FA was anchored by simple physical adsorption. Nanoparticles were freeze dried using a combination of trehalose and lutrol-f-68 as cryoprotectant. Nanoparticles with adequate drug loading and particle size 350-450nm were developed and found to be stable as per ICH guidelines. Nanoparticles were characterized for zeta potential, hydrophobicity, SEM, FTIR, DSC, pXRD, in vitro drug release etc. Nanoparticles revealed high uptake in human macrophage cells U-937 which further increased with folate anchoring.
Pagination: 189p.
Appears in Departments:Department of Pharmaceutical Sciences and Technology

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01_title.pdfAttached File35.87 kBAdobe PDFView/Open
02_certificate.pdf1.13 MBAdobe PDFView/Open
03_dedication.pdf34.92 kBAdobe PDFView/Open
04_acknowledgements.pdf49.89 kBAdobe PDFView/Open
05_declaration.pdf11.2 kBAdobe PDFView/Open
06_list of publication.pdf31.33 kBAdobe PDFView/Open
07_list of abbreviations.pdf11.76 kBAdobe PDFView/Open
08_list of figures.pdf18.97 kBAdobe PDFView/Open
09_list of tables.pdf19.72 kBAdobe PDFView/Open
10_abstract.pdf23.38 kBAdobe PDFView/Open
11_index.pdf14.46 kBAdobe PDFView/Open
12_part 1.pdf5.27 MBAdobe PDFView/Open
13_part 2.pdf2.95 MBAdobe PDFView/Open
14_part 3.pdf4.08 MBAdobe PDFView/Open
15_conclusion.pdf22.44 kBAdobe PDFView/Open
16_references.pdf166.68 kBAdobe PDFView/Open

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